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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as items (Fig. 9) - UV/Vis/NIR. As attracted Fig - https://calendly.com/olisclarity1/30min. 9, the response may also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As more shown in Fig. 9, this might be since the H4B extreme serve as an electron sink in a later point in the reaction, so regarding allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.